Phytomedicine. 2016 Dec 1;23(13):1583-1590. doi: 10.1016/j.phymed.2016.09.009. Epub 2016 Sep 28.

Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process.

Bueno-Silva B1, Franchin M2, Alves CF3, Denny C2, Colón DF4, Cunha TM4, Alencar SM5, Napimoga MH6, Rosalen PL2.

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Abstract

BACKGROUND:

Brazilian propolis is popularly used as treatment for different diseases including the ones with inflammatory origin. Brazilian red propolis chemical profile and its anti-inflammatory properties were recently described however, its mechanism of action has not been investigated yet.

AIM:

Elucidate Brazilian red propolis major pathways of action on the modulation of neutrophil migration during the inflammatory process.

METHODS:

The ethanolic extract of propolis (EEP) activity was investigated for neutrophil migration into the peritoneal cavity, intravital microscopy (rolling and adhesion of leukocytes), quantification of cytokines TNF-α, IL-1β and chemokines CXCL1/KC, CXCL2/MIP-2, neutrophil chemotaxis induced by CXCL2/MIP-2, calcium influx and CXCR2 expression on neutrophils.

RESULTS:

EEP at 10mg/kg prevented neutrophil migration into peritoneal cavity (p < 0.05), reduced leukocyte rolling and adhesion on the mesenteric microcirculation (p < 0.05) and inhibited the release TNF-α, IL-1β, CXCL1/KC and CXCL2/MIP-2 (p < 0.05). EEP at 0.01, 0.1 and 1µg/ml reduced the CXCL2/MIP-2-induced neutrophils chemotaxis (p < 0.05) without affect cell viability (p > 0.05).EEP at 1µg/ml decreased the calcium influx induced by CXCL2/MIP-2 (p<0.05). On the other hand, none of EEP concentrations tested altered CXCR2 expression by neutrophils (p>0.05).

CONCLUSION:

Brazilian red propolis appears as a promising anti-inflammatory natural product which mechanism seems to be by reducing leukocyte rolling and adhesion; TNF-α, IL-1β, CXCL1/KC and CXCL2/MIP-2 release; CXCL2/MIP-2-induced chemotaxis and calcium influx.

Copyright © 2016 Elsevier GmbH. All rights reserved.

PMID: 27823622 DOI: 10.1016/j.phymed.2016.09.009

[Indexed for MEDLINE]

 

* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.