Arch Oral Biol. 2016 Feb 3;65:77-81. doi: 10.1016/j.archoralbio.2016.02.001. [Epub ahead of print]
Influence of a Brazilian wild green propolis on the enamel mineral loss and Streptococcus mutans’ count in dental biofilm.
Cardoso JG1, Iorio NL2, Rodrigues LF2, Couri ML2, Farah A3, Maia LC1, Antonio AG4.
This study investigated the anti-demineralizing and antibacterial effects of a propolis ethanolic extract (EEP) against Streptococcus mutans dental biofilm.
Blocks of sound bovine enamel (n=24) were fixed on polystyrene plates. S. mutans inoculum (ATCC 25175) and culture media were added (48h-37°C) to form biofilm. Blocks with biofilm received daily treatment (30μL/1min), for 5 days, as following: G1 (EEP 33.3%); G2 (chlorhexidine digluconate 0.12%); G3 (ethanol 80%); and G4 (Milli-Q water). G5 and G6 were blocks without biofilm that received only EEP and Milli-Q water, respectively. Final surface hardness was evaluated and the percentage of hardness loss (%HL) was calculated. The EEP extract pH and total solids were determined. S. mutans count was expressed by log10 scale of Colony-Forming Units (CFU/mL). One way ANOVA was used to compare results which differed at a 95% significance level.
G2 presented the lowest average %HL value (68.44%±12.98) (p=0.010), while G4 presented the highest (90.49%±5.38%HL) (p=0.007). G1 showed %HL (84.41%±2.77) similar to G3 (87.80%±6.89) (p=0.477). Groups G5 and G6 presented %HL=16.11%±7.92 and 20.55%±10.65; respectively (p=0.952). G1 and G4 differed as regards to S. mutans count: 7.26±0.08 and 8.29±0.17CFU/mL, respectively (p=0.001). The lowest bacterial count was observed in chlorhexidine group (G2=6.79±0.10CFU/mL) (p=0.043). There was no difference between S. mutans count of G3 and G4 (p=0.435). The EEP showed pH 4.8 and total soluble solids content=25.9 Brix.
The EEP seems to be a potent antibacterial substance against S. mutans dental biofilm, but presented no inhibitory action on the de-remineralization of caries process.
Copyright © 2016 Elsevier Ltd. All rights reserved.
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.