Protective Effect of Propolis Extract on Pancreatic β Cell under Oxidative Stress in vitro
Sebastián Zagmutt1, 2, Elba Leiva3, Verónica Mujica4, Sergio Wehinger4,
1Master Program in Biomedical Sciences, Faculty of Health Sciences, Universidad de Talca
2Faculty of Health Sciences, Department of Biomedical Sciences, University of Talca
3Faculty of Health Sciences, University of Talca, Interdisciplinary Excellence Research Program Healthy Ageing (PIEI-ES), Talca, Chile
4Faculty of Medicine, Universidad Católicadel Maule, Talca, Chile
Introduction: Oxidative stress is one of the most important mechanisms in the emergence of type 2 diabetes. It would therefore be important to increase the antioxidant potential to prevent the deleterious effects of oxidative stress. Methods: MTT assay was performed to assess cell viability in the murine β TC-6 beta cell line. TBARs (thiobarbituric acid reactive substances) and GSH (glutathione) were measured and apoptosis were assessed by flow cytometry. Results: Exposure to 150 µM of H2O2 and 100 µM of tert-butyl hydroperoxide (t-BOOH) significantly reduced cell viability. When cells were simultaneously incubated with propolis extract (PE) and oxidants, cell viability relative to control was maintained. Exposure of cells to oxidants increased TBARs levels and reduced GSH concentration, a condition that was reversed when incubated with PE. A significant increase in apoptotic cells was seen when exposed to oxidants, however simultaneous incubation with PE reduced the number of apoptotic cells. Conclusion: PE has a protective effect against oxidative stress.
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