Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells*

Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells

Han Bit Kim and Byung Sun Yoo

Dept. of Life Science, Kyonggi University, Suwon, Korea

(Received June 7, 2016; Revised June 15, 2016; Accepted June 16, 2016)

Propolis is a multicomponent, active, complex resinous substance collected by honeybees from a variety of plant sources. We have studied the effect of propolis on neurite outgrowth of SH-SY5Y human neuro- blastoma cells induced to differentiate by all-trans-retinoic acid (RA). Propolis, at a concentration of 3 μg/ mL, had no significant effect on the viability of differentiating SH-SY5Y cells. However, the neurite out- growth of the differentiating SH-SY5Y cells treated with propolis (0.3~3 μg/mL) for 48 hr was signifi- cantly inhibited in a dose-dependent manner. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.3 to 3 μg/mL propolis resulted in decreased level of transglutaminase and 43-kDa growth-associ- ated protein (GAP-43) in a dose-dependent manner. The results indicate that propolis is able to inhibit neu- rite outgrowth of differentiating SH-SY5Y cells.

Full Paper:  Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells