J Agric Food Chem. 2017 Aug 11. doi: 10.1021/acs.jafc.7b02880. [Epub ahead of print]
Caffeic Acid Phenethyl Ester (Propolis Extract) Ameliorates Insulin Resistance by Inhibiting JNK and NF-κB Inflammatory Pathways in Diabetic Mice and HepG2 Cells Models.
Nie JR, Chang YN, Li Y, Zhou Y, Qin J, Sun Z, Li H.
Caffeic acid phenethyl ester (CAPE), extracted from propolis, was evaluated the ameliorative effects on insulin resistance and identified the mechanisms, using noninsulin-dependent diabetes mellitus (NIDDM) model mice and insulin resistance (IR) model cells. After 5-week of CAPE supplementation, insulin sensitivity, hyperlipidemia, and peroxisome proliferator-activated receptor-α (PPAR-α) levels were improved in mice. Pro-inflammatory cytokines in serum and the expressions of tumour necrosis factor- alpha (TNF-α) mRNA in tissues were markedly down-regulated from CAPE-treated mice. In vitro, CAPE supplement significantly improved glucose consumption, glucose uptake, glycogen content, and oxidative stress and decreased expression of glucose-6-phosphatase (G6Pase) mRNA in cells. Both in vivo and vitro, CAPE enhanced p-Akt (Ser473) and p-insulin receptor substrate (IRS)-1 (Tyr612), but inhibited p-JNK (Thr183/Tyr185), p-NF-κB p65 (Ser536) and nuclear translocation of p-NF-κB p65 (Ser536). In summary, CAPE can ameliorate on insulin resistance through modulation of JNK and NF-κB signaling pathway in mice and HepG2 cells.
PMID: 28799756 DOI: 10.1021/acs.jafc.7b02880
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