Arch Oral Biol. 2016 Feb 3;65:77-81. doi: 10.1016/j.archoralbio.2016.02.001. [Epub ahead of print]
Cardoso JG1, Iorio NL2, Rodrigues LF2, Couri ML2, Farah A3, Maia LC1, Antonio AG4.
This study investigated the anti-demineralizing and antibacterial effects of a propolis ethanolic extract (EEP) against Streptococcus mutans dental biofilm.
Blocks of sound bovine enamel (n=24) were fixed on polystyrene plates. S. mutans inoculum (ATCC 25175) and culture media were added (48h-37°C) to form biofilm. Blocks with biofilm received daily treatment (30μL/1min), for 5 days, as following: G1 (EEP 33.3%); G2 (chlorhexidine digluconate 0.12%); G3 (ethanol 80%); and G4 (Milli-Q water). G5 and G6 were blocks without biofilm that received only EEP and Milli-Q water, respectively. Final surface hardness was evaluated and the percentage of hardness loss (%HL) was calculated. The EEP extract pH and total solids were determined. S. mutans count was expressed by log10 scale of Colony-Forming Units (CFU/mL). One way ANOVA was used to compare results which differed at a 95% significance level.
G2 presented the lowest average %HL value (68.44%±12.98) (p=0.010), while G4 presented the highest (90.49%±5.38%HL) (p=0.007). G1 showed %HL (84.41%±2.77) similar to G3 (87.80%±6.89) (p=0.477). Groups G5 and G6 presented %HL=16.11%±7.92 and 20.55%±10.65; respectively (p=0.952). G1 and G4 differed as regards to S. mutans count: 7.26±0.08 and 8.29±0.17CFU/mL, respectively (p=0.001). The lowest bacterial count was observed in chlorhexidine group (G2=6.79±0.10CFU/mL) (p=0.043). There was no difference between S. mutans count of G3 and G4 (p=0.435). The EEP showed pH 4.8 and total soluble solids content=25.9 Brix.
The EEP seems to be a potent antibacterial substance against S. mutans dental biofilm, but presented no inhibitory action on the de-remineralization of caries process.
Copyright © 2016 Elsevier Ltd. All rights reserved.
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.