Akhmad Endang Zainal Hasan, Agus Setiyono, Miko Afrian
ABSTRACT
Negative effects on health will occur in excessive use of paracetamol. The main objective of this study was to determine the hepatoprotective activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats induced by paracetamol. The study was conducted on 36 Sprague Dawley rats with 6 rats in each group. Group 1 (normal control), group 2 (positive control, temulawak 42.86 mg/kg BW), group 3 (negative control), group 4 (infusion of Myrmeleon 10 mg/kg BW), group 5 (propolis extract 0.02 ml/kg BW), and group 6 (Hibiscus sabdariffa infusion 0.02 ml/kg BW). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations were determined spectrophotometrically and microscopically for liver histopathology. The results showed that during three weeks of treatment with Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats, this treatment could decrease ALT and AST activity as the positive control. The ALT activity values of the three materials were 69.26, 51.00, 51.00, 43.67, U/I with AST values of 110.29, 97.33, 91.00, 91.33 U/I. Based on Duncan’s test, there were significant differences between all treatments with negative and positive control groups. Likewise, with the histopathological scoring results, the negative control was found to be significantly different from all treatments. Therefore, treatment with propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. may have hepatoprotective benefit.
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.