J Ethnopharmacol. 2017 Jun 14. pii: S0378-8741(17)30743-2. doi: 10.1016/j.jep.2017.06.015. [Epub ahead of print]
Bueno-Silva B1, Kawamoto D2, Ando-Suguimoto ES2, Casarin RCV3, Alencar SM4, Rosalen PL5, Mayer MPA2.
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Abstract
ETHNOPHARMACOLOGICAL RELEVANCE:
Propolis has been used in folk medicine since ancient times and it presented inhibitory effect on neutrophil recruitment previously. However, its effect on macrophage obtained from mice remains unclear.
AIM OF THE STUDY:
to demonstrate BRP effects on LPS activated peritoneal macrophage.
MATERIALS AND METHODS:
Peritoneal macrophages, obtained from C57BL6 mice and activated with LPS, were treated with 50 to 80µg/mL of crude extract of Brazilian red propolis (BRP) during 48hours. Cell viability, levels of NO, 20 cytokines and expression of 360 genes were evaluated.
RESULTS:
BRP 60µg/ml reduced NO production by 65% without affecting the cell viability and decreased production IL1α, IL1β, IL4, IL6, IL12p40, Il12p70, IL13, MCP1 and GM-CSF. Molecular mechanism beyond the anti-inflammatory activity may be due to BRP-effects on decreasing expression of Mmp7, Egfr, Adm, Gata3, Wnt2b, Txn1, Herpud1, Axin2, Car9, Id1, Vegfa, Hes1, Hes5, Icam1, Wnt3a, Pcna, Wnt5a, Tnfsf10, Ccl5, Il1b, Akt1, Mapk1, Noxa1 and Cdkn1b and increasing expression of Cav1, Wnt6, Calm1, Tnf, Rb1, Socs3 and Dab2.
CONCLUSIONS:
Therefore, BRP has anti-inflammatory effects on macrophage activity by reducing NO levels and diminished release and expression of pro-inflammatory cytokine and genes, respectively.
Copyright © 2017. Published by Elsevier B.V.
PMID: 28624363 DOI: 10.1016/j.jep.2017.06.015
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.