Bruno Bueno-Silva,† Severino M. Alencar,*,‡ Hyun Koo,§ Masaharu Ikegaki,∥ Gil V. J. Silva,à Marcelo H. Napimoga,# and Pedro L. Rosalen†
†Department of Physiological Science, Piracicaba Dental School, University of Campinas (UNICAMP), C.P. 52, 13414-903 Piracicaba, Sao! Paulo, Brazil
‡College of Agriculture “Luiz de Queiroz” (ESALQ), University of Sao! Paulo (USP), C.P. 9, 13418-900 Piracicaba, Sao! Paulo, Brazil §Center for Oral Biology, Eastman Department of Dentistry, and Department of Microbiology and Immunology, University of
Rochester Medical Center, Rochester, New York 14642, United States
∥Federal University of Alfenas, 37130-000 Alfenas, Minas Gerais, Brazil
àDepartment of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirao! Preto (FFCLRP), USP, 14040-901 Ribeirao! Preto, Sao! Paulo, Brazil
#Laboratory of Immunology and Molecular Biology, Sao! Leopoldo Mandic Institute and Research Center, Campinas, Sao! Paulo, Brazil
The objective of this study was to evaluate anti-inflammatory and antimicrobial activities of neovestitol and vestitol isolated from Brazilian red propolis (BRP). BRP ethanolic extract (EEP), neovestitol, and vestitol were evaluated by anti-inflammatory properties using a neutrophil migration assay. The antimicrobial activity was evaluated by minimal inhibitory and bactericidal concentrations (MIC and MBC) against Streptococcus mutans , Streptococcus sobrinus , Staphylococcus aureus , and Actinomyces naeslundii . Neovestitol, vestitol, and EEP inhibited neutrophil migration at a dose of 10 mg/kg. Regarding antimicrobial activity, neovestitol showed MICs ranging from <6.25 to 25-50 μg/mL and MBCs ranging from 25-50 to 50-100 μg/mL, while vestitol showed MICs ranging from 25-50 to 50-100 μg/mL and MBCs ranging from 25-50 to 50-100 μg/mL. Both isoflavonoids neovestitol and vestitol are consistent bioactive compounds displaying anti-inflammatory and antimicrobial activities that can strongly act in a low dose and concentration and have a promising potential to be applied in the pharmaceutical and food industries.
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.