Antioxidant Activities of Propolis collected from Different Regions of Morocco
Ouamani Abdelati, Bencharki Bouchaib, Nacera Dari, Hilali Lahoucine
Abstract
Propolis is a resinous substance collected by honeybees from various plant sources. The composition of propolis depends on time, vegetation, and the area of collection. The main objective of this study is to study the antioxidant Activities of Propolis from Different Regions of Morocco. To achieve this goal, the present work examined the antioxidant activity of propolis collected from 4 regions of Morocco: Agadir, Marrakech, Rabat and Settat. Ethanol extracts of propolis were prepared and evaluated for their antioxidant activities by; 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging. The investigation of the polyphenol and flavonoid contents was measured spectrophotometrically. 20 μg/ml of principal active extracted from each sample was analyzed. The percentage of DPPH degradation (I%) was different from region to other (Rabat: 87.96 %; Agadir: 76,66%; Settat: 65,77% and Marrakech: 49,46%). The total polyphenol contents was affected significantly by the collection site (Rabat: 241.66±, Agadir: 191.18±0.91, Settat: 127.22±2.61 and Marrakech: 77.89±1.91). The same finding was observed with flavonoids concentration, which was different from à site too other (Rabat: 91.48±1.47, Agadir: 70.93±2.26, Settat: 46.52±0.63 and Marrakech: 12.13±0.29). It was also observed that all samples of ethanolic extracts of propolis showed free radical-scavenging activity. The highest activities were found for samples from Rabat and Agadir. Also, a positive correlation between antioxidant activity and chemical composition (flavonoid and polyphenol) was detected.
* THESE STATEMENTS HAVE NOT BEEN EVALUATED BY THE FOOD AND DRUG ADMINISTRATION. THIS IS NOT INTENDED TO DIAGNOSE, TREAT CURE OR PREVENT ANY DISEASE.